神经科学博士候选人：斑马鱼前脑的大脑结构与感觉处理机制

该项目通过高分辨率成像与计算分析，探讨斑马鱼前脑的神经结构如何影响感觉处理机制。研究人员将利用电子显微镜和钙成像等技术，分析神经回路及其对感觉刺激的功能反应，从而揭示感觉处理机制的相关信息。

关于该职位： 我们正在寻找一位积极性极高的博士候选人，加入我们的研究小组，以探究斑马鱼前脑中感觉处理背后的神经结构机制。该项目将结合先进的成像技术与计算方法，揭示大脑回路的结构组织如何影响感觉信息处理过程。 您是否有动力迈向博士研究，开启充满机遇的职业生涯？作为我们的博士候选人，您将致力于完成博士学业，同时获得宝贵的经验，使您具备在高等教育与研究领域内外的进一步发展潜力。 关于该项目： 该项目旨在揭示大脑结构如何影响斑马鱼前脑中的感觉处理机制。由于斑马鱼具有光学透明性、遗传可操作性以及保守的脊椎动物神经组织特征，作为强大的脊椎动物模型生物，成功候选人将利用多种高分辨率成像技术，以前所未有的精度重建神经回路，并将结构组织与功能感觉反应联系起来。 具体而言，该项目将实现以下相互关联的目标： 3D电子显微镜重建技术：利用3D体积电子显微镜对斑马鱼前脑回路进行大规模、高分辨率的超微结构图谱重建与分析。通过先进的分割与重建软件包，将提取出突触连接性、细胞类型特征及亚细胞组织结构信息。 钙成像技术：在规定的感觉刺激实验条件下，采用双光子钙成像技术记录斑马鱼前脑的群体级神经活动。功能响应特性将与解剖学上确定的电路元件进行对应分析。 共聚焦显微镜与组织学观察：采用共聚焦显微镜、免疫组织化学、荧光原位杂交及组织清创技术，以研究斑马鱼前脑中的细胞类型、分子标记及投射模式。 解剖学重建：进行单神经元及群体水平的解剖学重建，以绘制连接模式，并明确前脑感觉回路的结构组织。 数据分析与计算方法：开发并应用计算流程来处理大规模影像数据集，整合结构数据与功能数据，并揭示感觉处理回路的组织规律。 该项目需要采用真正的多学科综合方法，结合实验与计算技术，以揭示大脑结构如何产生感觉功能的内在机制。 职位职责： 完成博士教育直至获得博士学位 在上述框架内开展高质量研究 参与学术论文的撰写与科普内容的传播 积极参与研究小组工作：卡夫利研究所的"[会员信息]"实验室 参与国际活动，如参加会议及/或到国外机构进行研究访问 为本科与硕士阶段的教学贡献力量 参与促进职业发展的活动（研讨会、期刊讨论会、实验室会议） 请做好在就业后工作职责发生变化的准备。

Required selection criteria: Hands-on experience with reconstructing 3D electron microscopy and associated data analytical software packages for image segmentation and neural reconstruction (e.g., CAVEclient, CloudVolume, Neuroglancer, or equivalent). Hands-on experience producing 3D visualizations, renderings, or animations of reconstructed neural structures or volumetric imaging data (e.g., using Blender or comparable 3D rendering software), to support scientific communication and dissemination of the project's findings. Experience with Python programming, including data analysis libraries (e.g., NumPy, SciPy, etc.) and/or image processing packages. Proficiency in scripting reproducible analysis pipelines is expected. Practical experience with confocal microscopy and standard histological techniques, including immunohistochemistry, fluorescence labeling, and/or tissue clearing methods. Experience with anatomical reconstructions of neural circuits, including tracing of axonal projections, dendritic arbors, or synaptic connectivity, from either light-microscopy or electron-microscopy datasets. Experience with zebrafish husbandry, including routine maintenance of zebrafish colonies, breeding, and basic larval and adult handling procedures. Excellent written and oral communication skills in English. You must have an academically relevant background in Neuroscience, or a closely related discipline. You must have a relevant Master's degree in neuroscience, biomedical sciences, biology, or equivalent. Your degree must include a major independent project (minimum 30 ECTS) equivalent to a master’s thesis. Master students can apply, but the master's degree must be obtained and documented before starting the position in July 2027. You must have a strong and relevant academic background from your previous studies and have an average grade from your Master's degree study, or equivalent education, which is equal to B or better compared to NTNU's grading scale . If you do not have letter grades from previous studies, you must have an equally good academic foundation. If you have a weaker academic background, you may be considered if you can document that you are exceptionally suited for a PhD education, for example through relevant work experience and/or peer-reviewed academic works. You must meet the requirements for admission to the faculty's Doctoral Programme, see Section 6-1 of the PhD regulations for more information. The appointment is to be made in accordance with NTNUs guidelines for recruitment positions for general criteria for the position. Preferred selection criteria: Experience with calcium imaging (two-photon) and associated data analysis of neural population activity in zebrafish or other vertebrate models. Co-authorship in a high quality peer-reviewed scientific publication or preprint. This is considered a major advantage and will be weighted strongly in the candidate assessment. Excellent oral presentation skills and experience presenting research at group meetings, workshops, or international conferences. Experience preparing scientific figures using Adobe Illustrator, Inkscape, or comparable graphic design software. Personal characteristics: To complete a doctoral degree (PhD), it is important that you are able to: Work independently and take initiative Work in a structured way, set goals, and make plans to achieve them Present and discuss your research with other professionals Get involved and contribute constructively with feedback Work constructively under pressure or in the face of adversity Show curiosity and a strong motivation for the subject Analyze data, assess different perspectives, and draw well-founded conclusions Be flexible and open to adjusting the plan for the project as needed. Emphasis will be placed on personal qualities. Diversity: Diversity is a strength, and at NTNU we aim to be an employer that reflects the diversity in society and that makes use of the potential of the population's collective skills. Our vision is Knowledge for a better world and our values ​​are creative, critical, constructive and respectful . We believe that an organization that is equal, diverse and gender-balanced is essential for us to achieve our goals. We strive to attract employees with different skills, life experiences and perspectives to contribute to even better problem solving of our societal mission in research and education. If you think this position is relevant and interesting, we encourage you to apply, regardless of gender, functional ability and cultural background, or whether you have been out of work for a period of time.

We offer: An exciting research position in a world-leading neuroscience institute with an important mission in society Developing tasks in a strong and international scientific environment Career guidance and follow-up throughout the PhD period Open and inclusive working environment with committed and collaborative colleagues Working capital to implement the project, including access to state-of-the-art imaging infrastructure Mentor programme as a new employee at NTNU As a public employee, favorable benefits as a member of the Norwegian Public Service Pension Fund (SPK) As a PhD Candidate at NTNU, you will have access to employee benefits . Salary and conditions: In the position of PhD Candidate, code 1017, your gross salary will normally be NOK 580 000,- per annum depending on qualifications and seniority. A 2% statutory contribution to the State Pension Fund is deducted from the salary. The employment period is three years for the doctoral work in addition to four months of career promotion work. (A minimum of three work years of the total term period must be dedicated to doctoral work.) For employment as a PhD Candidate, it is a prerequisite that you gain admission to the PhD programme within three months of your employment contract start date, and that you participate in an organized doctoral programme through out the period of employment. As an employee at NTNU, it is important that you keep yourself up to date with academic and organizational changes and adapt to them. For the necessary professional and social interaction, it is a prerequisite that you are physically present and available to the institution on a daily basis. The appointment is carried out in accordance with the principles of the State Employees Act , and Export control (legislation that regulates the export of knowledge, technology and services). Candidates who, after assessment of the application and attachments, are considered to bein conflict with the criteria in the latter act, will not be able to be employed.

关于申请： 附件必须随申请文件提交，因为这些文件是评估申请的基础。所有文件必须使用英语或斯堪的纳维亚语言。 请注意：申请将仅依据我们在申请截止日之前收到的材料进行评估。因此，请确保您的申请明确说明您的技能与经验如何符合上述标准。申请及所有附件必须通过 Jobbnorge.no 电子方式提交。如果您被邀请参加面试，必须根据要求携带经认证的证书和文凭副本。 申请必须包含： 学士和硕士阶段的成绩单与学位证书。如果您近期已提交硕士论文，但尚未获得学位证书，可以附上论文初稿；必须提供已完成学位的证明文件，才能开始任职。 个人简历（CV）（不超过2页），包括完整的发表成果清单。 动机信（最多1页）：一封说明您申请动机、科学兴趣的信件，以及您的背景与过往经验如何符合上述所需及优先选拔标准的文字。请具体阐述，并将您的经历直接与该职位所需的技能联系起来。 研究计划书（最多1页）：一份简洁的研究计划书，概述您针对本项目所描述的一项或多项科学目标将采取的研究思路。该计划书应体现您对相关方法的理解以及提出科学问题的能力。不必严格遵循项目的具体目标——基于项目范围产生的创新想法同样被欢迎。 如可获取：出版物副本和/或预印本（包括您作为共同作者参与的任何正在撰写的研究成果）。 两名具有相关学术背景的推荐人姓名及完整联系方式，以便其提供推荐信。 如您的全部或部分教育经历在国外完成，除其他高等教育资料外，还须附上证明全部学士和硕士教育经历的学习范围与质量的材料。如果您的院校使用“文凭补充说明”（大多数欧洲院校的通常做法），必须一并提交。所需材料的说明也可在此处查看。如果您已取得挪威高等教育与技能局（HK-dir）出具的证明，也请附上。共同完成的研究成果也会纳入考虑。如难以判断您对共同研究的贡献，须另附简要说明，介绍您的参与情况。 在评估最符合条件的候选人时，我们重点关注教育背景、实践经验及个人适配性等必要条件。 申请该职位动机、抱负及研究潜力也将被纳入对申请人的评估之中。 挪威科技大学认可多种学术贡献，并承诺遵循《旧金山研究评估宣言》及CoARA原则。